After the Major Bowes tour ended, Berner transitioned to network radio in Hollywood, with recurring roles on Fibber McGee & Molly and The George Burns and Gracie Allen Show. On The Jack Benny Program, she played various one-time parts before joining the principal cast as the recurring characters of Jack Benny's girlfriend Gladys Zybisco, and wisecracking telephone operator Mabel Flapsaddle, who gossiped about Benny with her cohort Gertrude Gearshift (Bea Benaderet), while Benny waited impatiently on the other end of the line for them to complete his call. Intended as a one-time appearance, the pair became a recurring role starting in the 1945-46 season, and in early 1947, Berner and Benaderet momentarily took over the actual NBC switchboards in Hollywood for publicity photos. Other radio work included waitress Dreamboat Mulvany on Arthur's Place; Mrs. Horowitz on Life with Luigi; Helen Wilson on Amos 'n' Andy; and an Italian housekeeper on The Jimmy Durante Show. She was cast alongside Rudy Vallée on his show The Fleischmann's Yeast Hour; however, she sued Vallée in 1945 for $19,500 in damages over claims he reneged on an "oral agreement" that he would hire her for 39 appearances on his show at $500 weekly.