Ames appeared in a featured role in The Postman Always Rings Twice (1946) as district attorney Kyle Sackett. He also appeared in the Doris Day-Gordon MacRae film On Moonlight Bay, its sequel, By the Light of the Silvery Moon, and Peyton Place (1957). He played the role of Samuel Eaton, Alfred Eaton's (Paul Newman) father, in From the Terrace (1960). He appeared in the 1961 Walt Disney comedy The Absent-Minded Professor as Medfield College President Rufus Daggett and in the 1963 sequel, Son of Flubber. In 1970, he played Secretary of the Navy Frank Knox in the action war film Tora! Tora! Tora! His last film role was in Peggy Sue Got Married (1986), as Kathleen Turner's character's grandfather Barney Alvorg.